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A1R Confocal Laser Scanning Microscope

A1R Confocal Laser Scanning Microscope

A1R Confocal LSM

[Image Source: Nikon]                        

The Nikon NIE upright confocal microscope is part of the Johnstone Lab and is equipped for in vivo, live cell and fixed stained sample imaging. It is located in Room 2313C, 4 Riverside Circle.


  • A1-SHRM-C A1 R MP scan head and controller
  • Resonance scanner which can image up to 700fps
  • A1-DU4G HD detector with 4 channel PMT (2 GAASP high sensitivity PMT; 2 PMT)
  • A1-DUS 32 channel spectral scanner
  • Water immersion lenses for intravital, 10x 0.3 (W.D. 3.5mm), 40x Near Infrared Water Dipping Objective lens, N.A. 0.8 (W.D. 3.5mm)
  • Multiphoton LWD lens 25xMP N.A.1.1 (1.43-2.04mm)
  • Multi immersion lenses (oil, glycerin, water) 60x N.A.1.4, 20x N.A. 0.75
  • FTP-2000 motorized X/Y/Z platform with 150mm of Z plane
  • Piezo Focusing Element for Nikon FN1
  • Software:  NIS-ELEMENTS MODULE: RATIO & FRET Ratio: Ratio View, Live Ratio Graphing & Measurement, Calcium & New Titration Calibration FRET: Capture and Analysis Available for C, HC & AR only

Offline Wokstation

  • Enhanced Imaging Workstation: HP Z4 featuring Intel Xeon 3.6 GHzprocessor (4 cores), 32GB DDR4 2666 Mhz RAM, NVIDIA RTX 4000 8GB, 512GBTurbo M.2 OS drive, 2x2TB HDD storagE
  • NIS-Elements Advanced Research Stand Alone License
  • NIS.AI MODULE: NIS-Elements module for artificial intelligence and deep learning using convolutional neural networks (CNNs) to learn from training data to apply repeatedly to volumes of data
  • NIS-ELEMENTS MOD: GEN ANALYSIS 2 AND 3 modules.  Image processing and measurement analysis engine for custom and standard routines with support of ND dimensions, 3D features, Javascript support, output controls and many data management functions. This module includes both General Analysis 2 (GA2) and GA3. 
  • LIM 2D/3D deconvolution Suite: 2D Decon, 2D Fast Decon, 3D Blind, 3D PSF, 3DWiener
  • DUAL SCREEN – 28” Touchscreen and 31.5” Z32 4K UHD display


Scott Johnstone, Ph.D.