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Seahorse Real-Time Cell Metabolic Analysis

Seahorse Real-Time Cell Metabolic Analysis

Aligent Seahorse XFe96

[Image Source: Agilent]                        

The Seahorse facility for analysis of oxygen rate and proton efflux rate of live cells is located in Room R2097, 2 Riverside Circle, and is part of the LaMantia Lab.

Aligent Seahorse XFe96 Analyzer


  • 96-well plate format accommodates many conditions in a single run, for flexible assay design, dose-response studies, and screening.
  • Reports real-time metabolic rates in minutes, without sample extraction or labelling.
  • Four-port injection system with automated mixing function, for detection of live-cell responses to substrates, inhibitors, and other compounds in real time.
  • High-sensitivity analysis of as few as 5,000 cells per well.
  • Precision-controlled heating tray, maintains 16–42°C temperatures (12–20°C above ambient conditions), for compatibility with a variety of sample types.
  • Quickly determine dependency of cellular energy production on mitochondrial substrates.
  • Generate a metabolic phenotype within one hour.
  • Analyze 3D samples such as spheroids and islets.


Facility director
Daniel Meechan, Ph.D.