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Cell Sorting

Cell Sorting

Hgh parameter flow cytometry

Cell Sorter

Becton Dickinson FACSymphony S6 Cell Sorter

Flow Cytometry

Six-way cell sorter with five lasers, six detectors per laser line. Performs multicolor flow cytometry with up to 23 colors simultaneously. 

  • UV: 349-60
  • Violet: 405-200
  • Blue: 488-150
  • Green: 561-150
  • Red: 637-140

BSL-3 rated Class II, Type A2 Biosafety Cabinet with AMS

Refrigerated water circulator

Sorting into plates (support up to 1,536-well), slides, and most standard-sized centrifuge tubes

Spectral Analysis

  • Perform multicolor flow cytometry with up 23 colors simultaneously
  • Spectral flow can identify and quantify fluorescent signals even if they have substantial overlapping emission spectra
  • 6 detectors per laser line

All charges are per hour

INTERNAL USERS

Internal (12990)

  • O&M Fund (230284):  $500
  • Equipment Replacement Fund (230285): $0
  • Internal Total: $500

EXTERNAL USERS

External (12997)

  • O&M Fund (230284): $500
  • Equipment Replacement Fund (230285): $0
  • Subtotal: $500

External (12920)

  • O&M Fund (230284): $0
  • Equipment Replacement Fund (230285): $305
  • Subtotal $305
  • External Total: $805

Access is currently restricted to ABSL3 facility staff who will undertake required work.

  • To utilize the facility, interested faculty should contact facility director Michael Zeitz and facility faculty director James Smyth to describe proposed work and discuss feasibility.
  • Work will be scheduled and undertaken by facility staff.
  • Samples may be inactivated and rendered BSL-2 for further processing/analysis by researchers outside of the ABSL3 facility if needed.
  • All work undertaken requires Fralin Biomedical Research Institute ABSL3 staff involved be included as authors on any publication and given appropriate effort on grant proposals arising from work.

Please note: Anyone planning to use this facility must coordinate with the facility director. Access to the third floor of 4 Riverside Circle is restricted.

ABSL3 Lab: Room 3120, 4 Riverside Circle, Roanoke, VA

Parking at Fralin Biomedical Research Institute: During business hours, visitors may park in designated visitor parking places at the entrance to the parking garage at 2 Riverside Circle. Visitors may also park on the 2nd - 6th floors of the parking garage located at 5 Riverside Circle. On nights and weekends, visitors may park anywhere in the 5 Riverside parking garage, as well as the surface lot and parking deck. All parking is free.

Contacts

Facility director
Michael Zeitz, Ph.D.
mjzeitz@vtc.vt.edu
540-526-2239

Facility Faculty Director
James Smyth, Ph.D. 
smythj@vtc.vt.edu
540-526-2238