Animal Biosafety Level 3 Facility

A phase contrast and fluorescence microscope. Live- and fixed-imaging in brightfield/phase contrast and equipped with LEDs for fluorescence imaging in Ex: 357/44 Em: 447/60, Ex: 470/22 Em: 525/50, and Ex: 585/29 Em: 628/32. Imaging of live and actively infected preparations can be easily and safely performed on this system with 4x, 10x, 20x, 40x, and 100x objectives.

Multimode microplate reader capable of absorbance, fluorescence, and luminescence measurement from multiwall plates. Live and actively infected preparations can easily and safely be used in this instrument.

Permits sectioning of fixed and unfixed tissues from infected animals.

Rodent housing unit allows for coronavirus infection models and associated necessary containment.

Six-way cell sorter with five lasers, six detectors per laster line. Performs multicolor flow cytometry with up to 23 colors simultaneously. 

High-speed resonant scanning microscope allows for imaging up to 720 fps @ 2048x16. Scope is an AX-R 2K scan head and controller on a Ti2 motorized inverted microscope with 20x, 60x, and 100x objectives. The confocal side of the system offers four detectors and 405/488/561/640 lasers. The 4-channel DUX-ST detector allows up to 12 discreet bandpasses of emission.

The Incucyte S3 provides automatic live cell imaging and real time analysis, housed within the cell culture incubator. The microscope features 4X, 10X, and 20X objectives on an automated turret, two-color green|red fluorescent channels [Green (Ex: 440-480 nm, Em: 504-544 nm), Red (Ex: 565-605 nm, Em: 625-705 nm)] & HD phase contrast imaging. The instrument is capable of imaging up to 6 microplates in parallel, and has support for over 700 vessels including flasks, dishes, slides, and microplates. Additionally, the instrument includes The Woundmaker accessory for quickly making homogenous wounds on a 96-well plate for invasion and migration assays.

The Vevo F2 ultrasound provides high-definition image processing, and is equipped with 3D & 4D imaging, B-mode imaging, and power doppler applications for use with small animals. The provided linear probe, UHF57x (57-25 MHz), provides high-resolution cardiovascular, abdominal, reproductive, and tumor imaging (<14 mm diameter). 4D mode imaging allows for high spatial resolution + high temporal resolution to allow researchers to visualize the heart from any angle over the entire cardiac cycle. Power doppler imaging is used in conjunction with 3D-mode to provide quantitative vascularity measurements, e.g., assessing tumor vascularity in cancer progression.

The Buxco® Small Animal Whole Body Plethysmography (WBP) instrument provides gold standard data collection for indirect respiratory measurements in conscious, freely moving subjects. This instrument is comprised of two 4-site systems, providing eight small animal sites for simultaneous WBP data collection.

The DSI Implantable Telemetry system provides in vivo physiologic wireless monitoring of freely moving animals. The eight-site system is integrated into our Allentown BCU-2 to allow for continuous monitoring. DSI has a selection of small animal implants for a range of studies including glucose monitoring, ECG, EEG and EMG, blood pressure, body temperature, and activity.

The TSE Phenomaster is a multi-modular platform that allows researchers to carry out synchronous and automated metabolic, behavioral and physiological monitoring of small animals. Currently, our system consists of 10 cages with modules to measure activity & sleep analysis, feeding & drinking behavior, indirect calorimetry, and body weight.