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Tissue Processing

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TISSUE PROCESSING

The Tissue Processing facility, located in Room 2305 in 4 Riverside Circle, provides histology services and instruction to all Fralin Biomedical Research Institute researchers. 

     

Equipment

  • Tissue processor for formalin fixed tissues, Tissue Tek
  • Tissue embedding station, Tissue Tek
  • Microtome, Leica HistoCore AUTOCUT, fully motorized
  • Cryostat, Leica CM1850
  • Vibratome for fixed and non-fixed tissue
  • Sliding Microtome, Leica SM2010 R
  • Automated stainer, Sakura HistoTek SL
  • Nikon Histology microscope with 4x, 10x, 20x, 40x, 60 lenses and image stitching capabilities for large tissues
  • Water baths, ovens, manual staining equipment

Special Stains

Stain

Application

Method
Hematoxylin and Eosin Tissue pathology and inflammation Automated
Safranin O Cartilage Automated
Giemsa Nucleated blood cells Automated
Immunohistochemical staining Investigator defined Manual
Periodic Acid Schiff (PAS) Basement membrane Manual
Oil Red O Adipose tissues Manual

M1 Cost of Attendance 2021-2022

  • Months in Calendar: 11
  • Year of Graduation: 2025
  • Tuition: $54,653
  • Fees - billed by school: $1,429
  • Board Exams - (Projected): 0
  • Textbooks and Equipment: $970
  • Medical and Dental Insurance (Estimated): $3,628
  • Residency Interviewing Costs: 0
  • Living Expenses: $14,839
  • Transportation Expenses: $3,223
  • Personal/Misc. Expenses: $3,586
  • Loan Fees: $474
  • COA Totals: $82,802

 

M2 Cost of Attendance 2021-2022

  • Months in Calendar: 12
  • Year of Graduation: 2024
  • Tuition: $54,653
  • Fees - billed by school: $1,454
  • Board Exams - (Projected): $645
  • Textbooks and Equipment: $430
  • Medical and Dental Insurance (Estimated): $3,628
  • Residency Interviewing Costs: 0
  • Living Expenses: $16,188
  • Transportation Expenses: $3,516
  • Personal/Misc. Expenses: $3,912
  • Loan Fees: $498
  • COA Totals: $84,924

 

M3 Cost of Attendance 2021-2022

  • Months in Calendar: 12
  • Year of Graduation: 2023
  • Tuition: $54,653
  • Fees - billed by school: $1,529
  • Board Exams - (Projected): $645
  • Textbooks and Equipment: $100
  • Medical and Dental Insurance (Estimated): $3,628
  • Residency Interviewing Costs: 0
  • Living Expenses: $16,188
  • Transportation Expenses: $3,516
  • Personal/Misc. Expenses: $3,912
  • Loan Fees: $498
  • COA Totals: $84,669

 

M4 Cost of Attendance 2021-2022

  • Months in Calendar: 11
  • Year of Graduation: 2022
  • Tuition: $54,653
  • Fees - billed by school: $1,319
  • Board Exams - (Projected): 0
  • Textbooks and Equipment: $100
  • Medical and Dental Insurance (Estimated): $3,628
  • Residency Interviewing Costs: $5000 (Travel) and $678 (Residency-related Applications)
  • Living Expenses: $14,839
  • Transportation Expenses: $3,223
  • Personal/Misc. Expenses: $3,586
  • Loan Fees: $474
  • COA Totals: $87,500

MRI/PET

  • Internal Rate O&M  230994-12990: $250
  • Internal Total (Banner Funds): $250
  • External Rate O&M  230994-12997: $250
  • External Rate OH 230994-12920: $150
  • External Total (Bursar’s Office): $400

MRI/PET Extended Run

  • Internal Rate O&M  230994-12990: $150
  • Internal Total (Banner Funds): $150
  • External Rate O&M  230994-12997: $150
  • External Rate OH 230994-12920: $90
  • External Total (Bursar’s Office): $240

MRI/PET Contrast ml

  • Internal Rate O&M  230994-12990: $57
  • Internal Total (Banner Funds): $57
  • External Rate O&M  230994-12997: $57
  • External Rate OH 230994-12920: $34.20
  • External Total (Bursar’s Office): $91.20

Micro PET/CT

  • Internal Rate O&M  230994-12990: $250
  • Internal Total (Banner Funds): $250
  • External Rate O&M  230994-12997: $250
  • External Rate OH 230994-12920: $150
  • External Total (Bursar’s Office): $400

Micro PET/CT Tracer mCi

  • Internal Rate O&M  230994-12990: $31
  • Internal Total (Banner Funds): $31
  • External Rate O&M  230994-12997: $31
  • External Rate OH 230994-12920: $18.60
  • External Total (Bursar’s Office): $49.60

Hematoxylin and Eosin

  • Application: Tissue pathology and inflammation
  • Method: Automated

Safranin O

  • Application: Cartilage
  • Method: Automated

Giemsa

  • Application: Nucleated blood cells
  • Method: Automated

Immunohistochemical staining

  • Application: Investigator defined
  • Method: Manual

Periodic Acid Schiff (PAS)

  • Application: Basement membrane
  • Method: Manual

Oil Red O

  • Application: Adipose tissues
  • Method: Manual

MRI/PET

  • Internal Rate O&M  230994-12990: $250
  • Internal Total (Banner Funds): $250
  • External Rate O&M  230994-12997: $250
  • External Rate OH 230994-12920: $150
  • External Total (Bursar’s Office): $400

MRI/PET Extended Run

  • Internal Rate O&M  230994-12990: $150
  • Internal Total (Banner Funds): $150
  • External Rate O&M  230994-12997: $150
  • External Rate OH 230994-12920: $90
  • External Total (Bursar’s Office): $240

MRI/PET Contrast ml

  • Internal Rate O&M  230994-12990: $57
  • Internal Total (Banner Funds): $57
  • External Rate O&M  230994-12997: $57
  • External Rate OH 230994-12920: $34.20
  • External Total (Bursar’s Office): $91.20

Micro PET/CT

  • Internal Rate O&M  230994-12990: $250
  • Internal Total (Banner Funds): $250
  • External Rate O&M  230994-12997: $250
  • External Rate OH 230994-12920: $150
  • External Total (Bursar’s Office): $400

Micro PET/CT Tracer mCi

  • Internal Rate O&M  230994-12990: $31
  • Internal Total (Banner Funds): $31
  • External Rate O&M  230994-12997: $31
  • External Rate OH 230994-12920: $18.60
  • External Total (Bursar’s Office): $49.60

MRI/PET

  • Internal Rate O&M  230994-12990: $250
  • Internal Total (Banner Funds): $250
  • External Rate O&M  230994-12997: $250
  • External Rate OH 230994-12920: $150
  • External Total (Bursar’s Office): $400

MRI/PET Extended Run

  • Internal Rate O&M  230994-12990: $150
  • Internal Total (Banner Funds): $150
  • External Rate O&M  230994-12997: $150
  • External Rate OH 230994-12920: $90
  • External Total (Bursar’s Office): $240

MRI/PET Contrast ml

  • Internal Rate O&M  230994-12990: $57
  • Internal Total (Banner Funds): $57
  • External Rate O&M  230994-12997: $57
  • External Rate OH 230994-12920: $34.20
  • External Total (Bursar’s Office): $91.20

Micro PET/CT

  • Internal Rate O&M  230994-12990: $250
  • Internal Total (Banner Funds): $250
  • External Rate O&M  230994-12997: $250
  • External Rate OH 230994-12920: $150
  • External Total (Bursar’s Office): $400

Micro PET/CT Tracer mCi

  • Internal Rate O&M  230994-12990: $31
  • Internal Total (Banner Funds): $31
  • External Rate O&M  230994-12997: $31
  • External Rate OH 230994-12920: $18.60
  • External Total (Bursar’s Office): $49.60

All charges are per hour

INTERNAL USERS

Internal (12990)

  • O&M Fund (230284):  $500
  • Equipment Replacement Fund (230285): $0
  • Internal Total: $500

EXTERNAL USERS

External (12997)

  • O&M Fund (230284): $500
  • Equipment Replacement Fund (230285): $0
  • Subtotal: $500

External (12920)

  • O&M Fund (230284): $0
  • Equipment Replacement Fund (230285): $305
  • Subtotal $305
  • External Total: $805

Automated

  • Hematoxylin and Eosin (Tissue pathology and inflammation)
  • Safranin O (Cartilage)
  • Giemsa (Nucleated blood cells)

Manual

  • Immunohistochemical staining (Investigator defined)
  • Periodic Acid Schiff (PAS) (Basement membrane)
  • Oil Rd O (Adipose Tissues)
  • Begin by contacting either Scott Johnstone, Ph.D. (scottrj@vtc.vt.edu, 540-526-2296)
    or Clare Dennison (cldennison@vtc.vt.edu) to schedule a training session.
  • Training will be as long as needed, depending upon the user's familiarity with the equipment.
  • Once trained and approved, users can schedule time on the equipment. The facility director will provide calendar access. 
  • Users should supply their own slides, brushes, blades, and other consumables. If you lack these supplies, please contact the facility director.
  • Please also record equipment use in the log book next to each instrument.
  • Tissue processing and embedding
  • Paraffin-embedded and frozen tissue sections for:
    • Routine histology
    • Special stain
    • Immunohistochemistry
  • Microscopes for histology imaging, includes large image stitching capabilities

Contacts

Facility Faculty Director
Scott Johnstone, Ph.D. 
scottrj@vtc.vt.edu
540-526-2296

Facility Director
Clare Dennison, Ph.D.
cldennison@vtc.vt.edu