Protocols
Yan Lab Protocols
DNA Protocols
- Competent cells (DH5a and BL21)
- Competent cells (JM109)
- DNA isolation for generation of transgenic mice
- DNA isolation from agarose gel
- DNA isolation from acrylamide gel
- Ethanol precipitation of DNA
- Genomic DNA isolation
- Nanodrop for nucleic acid concentration
- Primer design for DNA sequencing
- Pyromark sequencing
- Recombinant DNA data sheet
- Site-directed mutagenesis
- Strategy for adding epitope tag
Blood protocols
Cell culture and tissue work protocols
- Ca2+ challenge assay for mitochondria
- Cytochrome c release from isolated mitochondria
- Glucose uptake assay in isolated muscles
- Live-dead assay by PI staining
- Mitochondrial isolation by centrifugation
- Mitochondrial respiration in C2C12 myotubes
- Mitochondrial triglyceride in skeletal muscle
- Tissue incubator maintenance
Imaging/microscopy
- Capillary density analysis
- EdU labeling and staining
- Gel image quantification
- Fiber type analysis
- Imaging MitoTimer in MitoTimer TG mice
- Image quantification using Scion Image
- Immunofluorescence for cultured myotubes
- Immunocytochemical labeling and FIRM
- Measure and calculate lung open space
- MitoTracker staining of cultured myotubes
- Oil Red O staining for skeletal muscle
- Oil Red O staining for cultured myotubes
- SDH staining for muscle sections
- TEM for cultured cells
- TEM for Drosophila
- TEM for tissues
- TEM image acquisition
- Tissue fixation for H&E
- Tissue whole mount immunostaining
- Western blot quantification by ImageJ
Human procedures
Bioinformatics
Reagents
- Antibody information
- Make ddH2O
- Solution Receipe and Protocols
Presentation and computer work
Animal procedures
- Anesthesia, glucose and insulin calculation
- Aurora muscle function protocol
- Baseline CLAMS
- BrdU injection
- Cardiotoxin injection
- Crossbreeding scheme
- CSQ mice breeding
- Dobutamine stress Echo
- Electrical stimulation parameters
- Echo machine right panel map
- EchoMRI body composition measurement
- Force transducer calibration
- Force transducer calibration for WBT
- Glucose tolerance test
- Glucose uptake in different tissues in vivo
- How to import/export mice
- Intramuscular cardiotoxin injection
- LPS injection for endotoxemia
- Measurement of O2 consumption for Drosophila
- Metabolic regulation
- Mouse BP
- Mouse CLP
- Mouse ECG
- Mouse Echo
- Mouse echocardiography (Yuntian)
- Mouse Echo Data Sheet
- Mouse Echo Calculation
- Parabiosis
- Sciatic Nerve Denervation
- Somatic gene transfer in FDB
- SOP for data colledtion and sample harvesting
- Streptozocin injection
- STZ injection (low dose)
- Tail cuff blood pressure
- Tamoxifen Injection
- Treadmill running (90 min)
- Treadmill power test using human treadmill
- Treadmill running test for mice
- Treadmill running test distance calculation table
- Treadmill running distance calculator
- VO2max test
- VO2max test (FBRI)
- Voluntary running for mice (DSI)
- Voluntary running for mice (ClockLab3)
- Weightlifting cage calibration
- Weightlifting cage set up table
- Weightlifting for mice
- Whole body tension test
- Whole body tension test calibration